9787030386069：分子克隆实验指南（第四版）

MolecularCloninghasservedasthefoundationoftechnicalexpertiseinlabsworldwidefor30years.Noothermanualhasbeensopopular，orsoinfluential.MolecularCloning，FourthEdition，bythecelebratedfoundingauthorJoeSambrookandnewco-author，thedistinguishedHHMIinvestigatorMichaelGreen，preservesthehighlypraiseddetailandclarityofpreviouseditionsandincludesspecificchaptersandprotocolscommissionedforthebookfromexpertpractitionersatYale，UMass，RockefellerUniversity，TexasTech，ColdSpringHarborLaboratory，WashingtonUniversity，andotherleadinginstitutions.Thetheoreticalandhistoricalunderpinningsoftechniquesareprominentfeaturesofthepresentationthroughout，informationthatdoesmuchtohelptrouble-shootexperimentalproblems.Forthefourtheditionofthisclassicwork，thecontenthasbeenentirelyrecasttoincludenucleic-acidbasedmethodsselectedasthemostwidelyusedandvaluableinmolecularandcellularbiologylaboratories.Corechaptersfromthethirdeditionhavebeenrevisedtofeaturecurrentstrategiesandapproachestothepreparationandcloningofnucleicacids，genetransfer，andexpressionanalysis.Theyareaugmentedby12newchapterswhichshowhowDNA，RNA，andproteinsshouldbeprepared，evaluated，andmanipulated，andhowdatagenerationandanalysiscanbehandled.Thenewcontentincludesmethodsforstudyinginteractionsbetweencellularcomponents，suchasmicroarrays，next-generationsequencingtechnologies，RNAinterference，andepigeneticanalysisusingDNAmethylationtechniquesandchromatinimmunoprecipitation.Tomakesenseofthewealthofdataproducedbythesetechniques，abioinformaticschapterdescribestheuseofanalyticaltoolsforcomparingsequencesofgenesandproteinsandidentifyingcommonexpressionpatternsamongsetsofgenes.Buildingonthirtyyearsoftrust，reliability，andauthority，thefourtheditionofMolecularCloningisthenewgoldstandard—theoneindispensablemolecularbiologylaboratorymanualandreferencesource.