INTERNATIONALTABLESFORCRYSTALLOGRAPHYVOLUMEF-CRYSTALLOGRAPHYOFBIOLOGICALMACROMOLECULES3E

InternationalTablesforCrystallographyVolumeFisanexpertguidetomacromolecularcrystallographyforthestructuralbiologist.ItwascommissionedbytheInternationalUnionofCrystallographyinrecognitionoftheextraordinarycontributionsthatknowledgeofmacromolecularstructurehasmade,andwillmake,totheanalysisofbiologicalsystems,fromenzymecatalysistotheworkingsofawholecell.Thevolumecoversallstagesofacrystallographicanalysisfromthepreparationofrecombinantproteins,throughcrystallization,diffractiondatacollection,phasedetermination,structurevalidationandstructureanalysis.Althoughthevolumeiswrittenforexperiencedscientists,itisrecognizedthatthereaderismorelikelytobeabiologistinterestedinstructurethanaclassicalcrystallographerinterestedinbiology.Thus,therearechaptersonthefundamentals,historyandcurrentperspectivesofmacromolecularcrystallography,aswellasonusefulprogramsanddatabasessuchastheProteinDataBank.Eachchapteriswrittenbyoneormoreinternationallyrecognizedexperts.Thissecondeditionfeatures19newarticlesandmanyarticlesfromthefirsteditionhavebeenrevised.Thenewarticlescovertopicssuchasstandarddefinitionsforqualityindicators,expressionofmembraneproteins,proteinengineering,high-throughputcrystallography,imagingofwholecells,radiationdamage,merohedraltwinning,low-resolutionabinitiophasing,roboticcrystalloading,whole-cellX-raydiffractionimagingandhalogeninteractionsinbiologicalcrystalstructures.Therearealsonewarticlesonrelevantsoftware,includingsoftwareforelectronmicroscopy.TheseenhancementswillensurethatVolumeFcontinuestobeakeyreferenceformacromolecularcrystallographersandstructuralbiologists.