ProteinMethods

ProteinMethodsDanielM.Bollag/MichaelD.Rozycki/StuartJ.EdelsteinReviewsfromthefirstedition"...itisverywellwritten...wouldcertainlybeofusetoundergraduateandpostgraduatestudentsenteringthecomplexworldofproteinpreparationandtomoreexperiencedscientistsenteringthisfieldforthefirsttime."–BiotechnologyandAppliedBiochemistry"Thebookpresentsthesetopicswithloadsofpracticaldetail,sothatthereaderhaslittleneedtoconsultotherreferencesourcestocarryoutthetechniquesdescribed.Allinall,ausefulbook..."–Theoretical&AppliedGenetics"...clearlywrittenwithprotocolsthatareeasytofollowandthetextiswellspread–outandeasytoread...allmethodsarefullyreferenced...ausefulbookforbeginnersatareasonableprice."–FEBSLettersThisrevisedandexpandedSecondEditionofProteinMethodsremainsthefirstsourceforacompletesummaryoftestedandprovenproteintechniques.Nowdividedintotwoparts,thebookbeginswiththeessentialchaptersfromthefirstedition,updatedtoreflectimportantchangesinmethodology.Thechapteronproteinisolationincludesanewsectionfocusingontheisolationofproteinsfrominclusionbodies.Inthesecondsection,fournewchaptersaredevotedtoproteinpurificationandcrystallization.Chaptersinclude?PreparationforProteinIsolation?ProteinExtractionandSolubilization?ProteinConcentrationDetermination?ConcentratingProteinSolutions?GelElectrophoresisUnderDenaturingConditions?GelElectrophoresisUnder...andfeature?reagentitemsandequipmentrequiredforeachmethod?chapter"view–at–a–glance"tabs?illustrationsforallgelapplicationsandothermethods?completesupplierlist?appendicesofbasicchemicalparametersandmoleculardata?keyreferences?descriptivetables?Safetynotes.