内容简介
Thisthoroughlyrevisedandupdatedthirdeditionprovidesanoverviewoftheprinciplesandmethodologyofthemostfrequentlyusedbiochemicalseparationtechniquesforproteinsusedtoday.Balancingtheory,procedures,andapplications,itoffersprofessionalsandstudentsinbiochemistry,organicchemistry,andanalyticalchemistryquickaccesstoawiderangeofimportanttechniques,including:GelfiltrationandionexchangechromatographyAffinityandcovalentchromatography?Reversedphaseandhydrophobicinteractionchromatography?Ligandsfromcombinatoriallibraries?Electrophoresisingels?Immunoelectrophoresisandcapillaryelectrophoresis?Two–dimensionalelectrophoresisinproteomicsProteinelutionandblottingtechniquesLarge–scaleelectrophoresisprocesses